The Design of a Quantitative Western Blot Experiment

Table 2

Computational analysis of densitometric data (*density data from a control sample loaded in the first lane of each gel/blot for a given study).

Biological replicates	Sample	Density (volume)
		Target protein (TP)	Loading control (LC)	Normalized density to LC (NDL) (TP* LC/LC*)	Fold difference (NDL/NDL*)
M1	𝑇=0	8	4*	(8×4/4)=8.0^∗	(8/8)=1.0
M1	𝑇=1	45	5	(45×4/5)=36.0	(36/8)=4.5
M1	𝑇=2	90	6	(90×4/6)=60.0	(60/8)=7.5
M2	𝑇=0	6	5	(6×4/5)=4.8	(4.8/8)=0.6
M2	𝑇=1	40	5	(40×4/5)=32.0	(32/8)=4.0
M2	𝑇=2	88	7	(88×4/7)=50.3	(50.3/8)=6.3
M3	𝑇=0	10	4	(10×4/4)=10.0	(10/8)=1.3
M3	𝑇=1	48	6	(48×4/6)=32.0	(32/8)=4.0
M3	𝑇=2	92	6	(92×4/6)=61.3	(61.3/8)=7.7