Nodal Promotes the Self-Renewal of Human Colon Cancer Stem Cells via an Autocrine Manner through Smad2/3 Signaling Pathway
Figure 5
Expression of CD24 and CD44, as well as Nodal and its receptors in CD44- and CD24-positive and -negative colon cancer cells. (a-b) Immunofluorescence showed the expression of CD24 (a) and CD44 (b) in the freshly isolated CD24- and CD44-positive HCT116 cells. (c-d) RT-PCR revealed transcripts of Nodal, ALK-4, ALK-7, and Actr-IIb in CD44-negative SW480 cells and CD44-negative LOVO cells (c) as well as in CD24-negative HCT116 cells (d). Gapdh was used as a loading control of total RNA. (e) RT-PCR displayed transcripts of Nodal, ALK-4, ALK-7, and Actr-IIb in CD24-positive HCT116 cells. Gapdh was used as a loading control of total RNA.
