Research Article
Nodal Promotes the Self-Renewal of Human Colon Cancer Stem Cells via an Autocrine Manner through Smad2/3 Signaling Pathway
Table 1
Primer sequences of genes for RT-PCR.
| Genes | Primer sequences | Product size (bp) | Tm (°C) |
| Nodal | Forward 5′-CCAAGCAGTACAACGCCTAT-3′ Reverse 5′-ACCCACATTCTTCCACGA-3′ | 234 | 51 |
| ALK-4 | Forward 5′-GCGTGTCTATCACAACCG-3′ Reverse 5′-TCAGCAGCAATAAATCCAA-3′ | 345 | 51 |
| ALK-7 | Forward 5′-ATAGCGGACTTAGGGTTG-3′ Reverse 5′-CTGGTTTGGGATACTTGG-3′ | 327 | 51 |
| Actr-IIb | Forward 5′-AGGCTGAGACACGGGAGT-3′ Reverse 5′-AGGCTGAGACACGGGAGT-3′ | 394 | 51 |
| Gapdh | Forward 5′-AATCCCATCACATCTTCC-3′ Reverse 5′-CATCACGCCACAGTTTCC-3′ | 382 | 55 |
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