Nodal Promotes the Self-Renewal of Human Colon Cancer Stem Cells via an Autocrine Manner through Smad2/3 Signaling Pathway

Table 1

Primer sequences of genes for RT-PCR.

Genes	Primer sequences	Product size (bp)	Tm (°C)
Nodal	Forward 5′-CCAAGCAGTACAACGCCTAT-3′; Reverse 5′-ACCCACATTCTTCCACGA-3′	234	51
ALK-4	Forward 5′-GCGTGTCTATCACAACCG-3′; Reverse 5′-TCAGCAGCAATAAATCCAA-3′	345	51
ALK-7	Forward 5′-ATAGCGGACTTAGGGTTG-3′; Reverse 5′-CTGGTTTGGGATACTTGG-3′	327	51
Actr-IIb	Forward 5′-AGGCTGAGACACGGGAGT-3′; Reverse 5′-AGGCTGAGACACGGGAGT-3′	394	51
Gapdh	Forward 5′-AATCCCATCACATCTTCC-3′; Reverse 5′-CATCACGCCACAGTTTCC-3′	382	55