Stimuli-Sensitive Hydrogel Based on N-Isopropylacrylamide and Itaconic Acid for Entrapment and Controlled Release of Candida rugosa Lipase under Mild Conditions

Table 2

The lipase velocity release constant and mesh sizes for the hydrogels with no itaconic acid swollen to equilibrium in solution of lipase concentration of 1 mg/mL. Each data point is calculated based on at least three measurements.

Sample	pH = 2.20 ± 0.01 (2 h) → pH = 6.80 ± 0.01 (24 h) at 37°C
	𝑘, h^−1	𝑛	𝑡1/2, h
95/5/2	—	—	6.82
100/0/2	0.022	1.02	20.86
95/5/4	—	—	4.05
100/0/4	0.018	1.05	19.28