High Molecular Weight Proteins of Trypanosoma cruzi Reduce Cross-Reaction with Leishmania spp. in Serological Diagnosis Tests
Figure 3
Electrophoresis of T. cruzi epimastigotes extracts. (a) SDS-PAGE of sonicated extract was performed in 12% polyacrylamide gel; each concentration has been pointed out. In all cases high molecular weight proteins (M.W. > 95 kDa) were found to be scarce. Gel was stained with Coomassie blue G250. (b) Electrophoresis of high molecular weight protein fractions of T. cruzi epimastigotes extracts. (1) Sonication. (2) Triton X-100 extraction. (3) NP40 extraction. (4) Urea-thiourea extraction. Concentration in each case was 15 μg. SDS-PAGE was performed in 6% polyacrylamide gels. Differences between extracts and their molecular weight are highlighted (black arrows). Molecular weight markers (Fermentas) were used (WM), and gel was stained with Coomassie blue G250 or Silver staining.
