Effect of Dietary 𝑛−3 Polyunsaturated Fatty Acids on Oxidant/Antioxidant Status in Macrosomic Offspring of Diabetic Rats

Table 4

In vitro oxidation of VLDL-LDL isolated from control and macrosomic rats.

	Day 0	Day 0	Day 90	Day 90
	Control	Macrosomic	Control	Macrosomic
Basal oxidation hydroperoxide (nmol/mg VLDL-LDL protein)
ISIO	15.29 ± 1.38	26.77 ± 1.05∗∗	27.50 ± 1.22	57.95 ± 2.17∗∗
EPAX	13.74 ± 1.02	19.94±1.11^∗§	25.95 ± 1.15	43.18±2.08^∗§§
Oxidation with CuSO4 Hydroperoxide (nmol/mg VLDL-LDL protein)
ISIO	46.51 ± 3.42	83.87 ± 4.36∗∗	79.94 ± 5.54	162.52 ± 8.48∗∗
EPAX	50.36 ± 2.33	102.96±5.25^∗§	90.27 ± 6.38	296.98±9.41^∗∗§§
Oxidation with CuSO4 TNBS (free lysine groups, %)
ISIO	−17.25 ± 1.04	−29.5 ± 1.26∗∗	−30.8 ± 2.40	−42.67 ± 2.35∗∗
EPAX	−15.16 ± 1.35	−27.6 ± 2.18∗∗	−31.35 ± 2.32	−45.98 ± 2.29∗∗

Values are mean ± SD; 𝑛=8 rats. Day 0: birth; day 90: adulthood; EPAX: EPAX diet; LDL: low density lipoprotein; ISIO: ISIO diet; TNBS: trinitrobenzene sulfonic acid assay; VLDL: very low density lipoprotein. Data were analysed statistically by ANOVA. Significant differences between macrosomic and control rats and between ISIO and EPAX diets at each age were assessed using Student’s 𝑡 test. ^∗𝑃<0.05 and ^∗∗𝑃<0.01 compared with the control group. ^§𝑃<0.05 and  ^§§𝑃<0.01 compared with the group fed with the ISIO diet.