Nerve Regenerative Effects of GABA-B Ligands in a Model of Neuropathic Pain
Figure 3
Morphological alterations of peripheral nerves are recovered after GABA-B ligands treatment. Toluidine blue stained semithin cross-sections of (a) sham operated rat. Evidence of normal structure of the nerve. (b) PSL rat in which a loss of myelinated fibers is evident. The nerve fiber structure appears degenerated and macrophages (indicated by arrowheads) are observed. (c) PSL rat treated with baclofen 10 mg/kg. Nerve appears still degenerated with a significant loss of myelinated fibers. (d) PSL rat treated with CGP56433 3 mg/kg. Nerve shows normal myelinated fibers, although some areas still contain degenerated axons (arrows). (e) PSL rat treated with baclofen + CGP56433. Nerve shows a regeneration pattern with several normal myelinated fibers and very few degenerated axons (arrows). Scale bar 20 μm. (f) Quantitative morphometric analysis of the number of myelinated fibers. All data are expressed as means ± SEM. Statistic with one-way Anova and Tuckey’s post hoc test. .
