Table 4: Multivariate logistic regression analysis and clinical response to methotrexate.

Genetic variablesAdjusted variables
Patient-related Patient-related + disease-related Patient-related + disease-related + treatment-related

valueOR (95% CI) value OR (95% CI) value OR (95% CI)

MTHFR C677T, rs1801133
 C carriersReferenceReferenceReference
 TT 0.0402.64 (1.04–6.67)0.0253.23 (1.16–9.02)0.0134.63 (1.37–15.60)

ATIC T675C, rs4673993
 CC ReferenceReferenceReference
 T carriers 0.0223.20 (1.18–8.66)0.0074.63 (1.51–14.12)0.0135.16 (1.42–18.76)

value < 0.05 is considered to be of statistical significance (highlighted in bold).
Adjusted variables include (1) patient-related variables (age, gender, and smoking), (2) disease-related variables (diagnosis age, disease duration, anti-CCPs, ANAs, TJC, SJC, and HAQ), and (3) treatment-related variables (folic acid supplementation, corticosteroids therapy, use of NSAIDs, other concomitant DMARDs used and MTX administration characteristics such as dose, treatment duration, and administration route). Genetic variables include MTHFR C677T and ATIC T675C polymorphisms.
ANAs: antinuclear antibodies; anti-CCP: anti-cyclic citrullinated peptide; ATIC: 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase; C: cytosine; CI: confidence interval; HAQ: health assessment questionnaire; MTHFR: methylenetetrahydrofolate reductase; NSAIDs: nonsteroidal anti-inflammatory drugs; OR: odds ratio; SJC: swollen joints count; T: thymine; TJC: tender joints count.