BioMed Research International / 2014 / Article / Fig 4

Research Article

Polymorphisms at Amino Acid Residues 141 and 154 Influence Conformational Variation in Ovine PrP

Figure 4

ELISA reactivity of metal ion-treated ovine recombinant PrP. Ovine recombinant PrP ALRQ (a, c, e, g) or ALHQ (b, d, f, h) was treated with water (black square) or either 0.2 mM (black circle) or 2 mM (black triangle) copper (a–d) or manganese (e–h) at 37°C as described in Section 2. Serial 2-fold dilutions of recombinant PrP were analysed by direct ELISA for reactivity with anti-PrP monoclonal antibody FH11 (a, b, e, f) or V47 (c, d, g, h). The data shown are the means of triplicate wells ± SD.
372491.fig.004a
(a)
372491.fig.004b
(b)
372491.fig.004c
(c)
372491.fig.004d
(d)
372491.fig.004e
(e)
372491.fig.004f
(f)
372491.fig.004g
(g)
372491.fig.004h
(h)

We are committed to sharing findings related to COVID-19 as quickly and safely as possible. Any author submitting a COVID-19 paper should notify us at help@hindawi.com to ensure their research is fast-tracked and made available on a preprint server as soon as possible. We will be providing unlimited waivers of publication charges for accepted articles related to COVID-19. Sign up here as a reviewer to help fast-track new submissions.