BioMed Research International / 2014 / Article / Tab 5

Review Article

Emerging Rapid Resistance Testing Methods for Clinical Microbiology Laboratories and Their Potential Impact on Patient Management

Table 5

Approximate turn-around-time, investment costs, reagent costs, and necessity of skilled personnel of different rapid diagnostic test procedures.

Rapid diagnostic procedureTurn-around-timeInvestment costsReagents costs
(per sample)
Necessity of skilled personnel

Agglutination assays<5 minutes<1.00€Low
Fluorescence in situ hybridization1-2 hours<15,000.00€1.00–8.00€Intermediate
Real-time PCR
(including DNA preparation)
4–6 hours35,000.00–60,000.00€15.00–25.00€Strongly depending on the test system
Loop-mediated isothermal amplification (LAMP) assays<1 hour2,000.00–4,000.00€15.00–25.00€Intermediate
Next generation sequencing (NGS)2–5 days350,000.00–750,000.00€75.00–800.00€Very high
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS)<5 minutes75,000.00–300,000.00€<1.00€High