Neutralizing Effects of Mimosa tenuiflora Extracts against Inflammation Caused by Tityus serrulatus Scorpion Venom
Figure 4
Effect of dichloromethane (CH2Cl2), ethyl acetate (EtOAc), and -butanol (-BuOH) fractions of M. tenuiflora against T. serrulatus venom-induced inflammation. BALB/c mice were treated intravenously (i.v.) with CH2Cl2 (JPF1), EtOAc (JPF2), and -BuOH (JPF3) fractions (a) at a dose of 40 mg/kg and a few minutes later were injected i.p. with 0.1 mL of VTs (0.8 mg/kg). After six hours, peritoneal lavage was performed with PBS and the cell number was determined in a Neubauer chamber. The supernatants were collected for determination of IL-6 (b), IL-12 (c), and IL-1 (d) levels, which was performed using an enzyme-linked immunosorbent assay. , , compared to PBS and drug-treated groups.
