Methylation-Associated Gene Silencing of RARB in Areca Carcinogens Induced Mouse Oral Squamous Cell Carcinoma
Methylation status in human oral cancer cell lines of RARB. (a) The schematic for CpG island presentation of RARB. There was a CpG island located in promoter region. We designed the MS-PCR and bisulfite sequencing primer sets in this region. (b) The RARB methylation status in different human oral cancer cell lines. The cell lines were also treated with 5′-aza-dC. The methylation status was recovered because of 5′-aza-dC treatment. (c) The RARB mRNA expression in different human oral cancer cell lines. The RARB was not expressed in Ca922, OC2, and HSC3 obviously. (d) The RARB bisulfite sequencing in different human oral cancer cell lines. There were more methylated RARB in TW2.6, Ca922, OC2, and HSC3 than in NHOK and DOK. The hollow circle is the unmethylated CpG site and the full circle is the methylated CpG site.