Angiotensin I-Converting Enzyme Inhibitor Derived from Cross-Linked Oyster Protein
Figure 5
Separation procedures of ACE inhibitory peptides by ion-exchange (a), size exclusion (b), and reversed-phase chromatography (c). Ion-exchange chromatography was performed with a linear gradient of 20 mM Tris-Cl containing 0.75 M NaCl, pH 8.0 on a HiLoad 16/10 Q-Sepharose column (16 × 100 mm) for 100 min at a flow rate of 1 mL/min. Size exclusion chromatography was performed with 20 mM Tris-Cl, pH 7.5 on a Superdex peptide (10 × 300 mm) column at a flow rate of 0.5 mL/min. Reversed-phase chromatography was performed on a Source 5RPC ST column (4.6 × 150 mm). Eluent A consisted of 0.1% TFA/water (v/v), and eluent B was 0.1% TFA/60% acetonitrile (v/v) with a linear gradient for 90 min at a flow rate of 1 mL/min.
