Complete Cell Killing by Applying High Hydrostatic Pressure for Acellular Vascular Graft Preparation

Figure 1

Schematic images of the evaluation of cellular viability with or without the pressure treatment. In the first step, the cell suspension was packed into the plastic bag. In the second step, the cells were treated with pressure. Finally, the cell killing was evaluated by cell morphology, WST-8 assay, and live/dead staining. The picture was cited from http://www.kobelco.co.jp/machinery/products/ip/product/cip/cip_05.html.