Complete Cell Killing by Applying High Hydrostatic Pressure for Acellular Vascular Graft Preparation

Figure 5

Evaluation of cell permeability by fluorescence images of UHP treated cells under live/dead staining after pressure treatment at 3 hours. (a–e) NIH/3T3, (f–j) smooth muscle, and (k–o) endothelial cells are shown as phase-contrast images. The cells were treated with the pressure of 0 (a, f, and k), 100 (b, g, and l), 200 (c, h, and m), 500 (d, i, and n), and 1000 (e, j, and o) for 10 min. The membrane permeability was disturbed with the pressure treatment of above 500 MPa.

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