KCTD11 Tumor Suppressor Gene Expression Is Reduced in Prostate Adenocarcinoma

Figure 3

KCTD11 inhibits cellular proliferation. (a) Q-RT-PCR analysis of KCTD11 mRNA expression in prostate cancer cell lines. (GAPDH was used as endogenous control.) Data are mean ± S.D. of triplicate wells. (b) BrdU-positive cells in KCTD11 overexpressing cell lines. Data are mean ± S.D. from three replicates (^∗𝑃<0.05; ^∗∗𝑃<0.01). (c) Representative images of BrdU immunofluorescence assay in prostatic cells stably transfected with KCTD11-GFP. (d) Western blot showing p21 and p27 expression levels in prostate cell lines overexpressing KCTD11. (e) Q-RT-PCR analysis of KCTD11 mRNA expression in pCXN2-human KCTD11 transfected prostate cancer cell lines. (GAPDH was used as endogenous control.) Data are mean ± S.D. of triplicate wells. (f) Q-RT-PCR analysis of Gli1, Patch1, IGF-2, and Cyclin D2 mRNA expressions in KCTD11 overexpressing cells. Data are mean ± S.D. of triplicate wells. (^∗𝑃<0.05; ^∗∗𝑃<0.01, and ^∗∗∗𝑃<0.005.)