Investigating hsp Gene Expression in Liver of Channa striatus under Heat Stress for Understanding the Upper Thermal Acclimation
Figure 1
hsp gene expression analysis in Channa striatus experimentally exposed to high temperature (36°C) and those collected from hot spring runoff. Real time RT-PCR analysis of hsp transcripts in liver tissues of the heat-stressed fishes (36°C for 4-, 15-, and 30-days) and those collected from the Atri hot spring runoff was carried out (marked Atri); (a) hsp70, (b) hsp78, (c) hsp60, (d) hsp90, (e) hsp110, (f) hsp47, (g) hsp27, and (h) tubulin (control). All data were expressed as mean standard error ( for each experimentally exposed group and for hot spring runoff collected fish) relative to control (Channa were collected from aquaculture ponds, water temperature 25°C and were maintained at 25°C in the laboratory), which was set as unit. Asterisks (*) denote statistically significant difference between control and experimentally exposed samples/fish collected from hot spring ().