Research Article

Functional Interactions between 17β-Estradiol and Progesterone Regulate Autophagy during Acini Formation by Bovine Mammary Epithelial Cells in 3D Cultures

Figure 1

E-cadherin localization in acinar structures formed by BME-UV1 cells cultured on Matrigel for 12 days in differentiation medium (control), enriched with 17β-estradiol (E2, 1 nM), progesterone (P4, 5 ng/mL), or both ( ); E-cadherin (epithelial cell marker) was labeled with antibodies conjugated with Alexa Fluor 488 (green fluorescence) and DNA was counterstained with 7AAD (red fluorescence).
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