Assessment of Antioxidant Activity of Spray Dried Extracts of Psidium guajava Leaves by DPPH and Chemiluminescence Inhibition in Human Neutrophils

Table 8

Analysis of the cytotoxicity of concentrated extract and spray dried extracts of Psidium guajava on stimulated neutrophils.

Samples	Viable cells (%)^c Trypan blue	LDH^d activity (U/mL)	Liberated LDH (% positive control)^e
Positive control^a	—	0.1516 (0.00)	100
Negative control^b	96.5 (0.71)	0.0126 (0.01)	8.40 (5.85)
DMSO control (0.2%)	97.0 (0.71)	0.0166 (0.002)	11.10 (1.55)
Conc. Ext.	94.00 (2.83)	0.0137 (0.02)	7.58 (8.76)
MA 80	94.2 (3.18)	0.0158 (0.01)	10.53 (3.68)
MDEA 80	96.7 (1.06)	0.0153 (0.001)	10.20 (0.47)
𝜷 CD 80	93.2 (1.06)	0.0166 (0.00)	11.07 (0.19)

Values are presented as the mean (standard deviation). ^a1 × 10⁶ neutrophils/mL lysed with 0.2% Triton X-100; ^b1 × 10⁶ neutrophils/mL incubated with Hanks; DMSO: solvent control; ^c% of viable cells, determined by Trypan blue exclusion assay, based on counts of 200 cells; ^dLDH enzyme activity, evaluated by the Liquiform (Labtest Diagnóstica) LDH kit; ^epercentage of LDH liberated in the supernatant compared with neutrophils completely lysed with Triton X-100.