Synergistic Effect of MiR-146a Mimic and Cetuximab on Hepatocellular Carcinoma Cells
Figure 12
Combination of miR-146a mimic and cetuximab induced more cleaved caspase-3 in HCC HepG2 cells. HepG2 cells (2.5 × 104 cells per well in 24-well plate) were cultured for 24 h then transfected with miR-146a mimic and negative control (200 nM). Meanwhile, cetuximab was added and the cells were cultured up to another 96 h. Western blot and signal intensity of the bands were shown. Antibodies include full length caspase-3, cleaved caspase-3, and β-Actin. M: mock control; C2: negative control for miRNA mimic; Mimi: miR-146a mimic; Cetu: cetuximab; comb: combination of miR-146a and cetuximab.
