Sequence-Based Appraisal of the Genes Encoding Neck and Carbohydrate Recognition Domain of Conglutinin in Blackbuck (Antilope cervicapra) and Goat (Capra hircus)

Figure 4

Alignment of predicted amino acid sequences of goat and blackbuck conglutinin NCRD with other wild and domestic ruminants (cattle, buffalo, sheep, and nilgai) and collectins (cattle collectin-43, cattle collectin-46, cattle surfactant protein-D, recombinant human surfactant protein D (rhSP-D), and recombinant rat surfactant protein A (rrSP-A)). Identity of the sequences is indicated by dots and the differences by corresponding one letter symbol of amino acids. Gaps introduced for optimal alignment are indicated by dashes. Four conserved cysteine residues are indicated by vertical (green) boxes. Predicted regions of secondary structures are indicated by horizontal (blue) boxes (A1-alpha helical region-1, A2-alpha helical region-2, A3-alpha helical region-3, B1-beta sheet region-1, B2-beta sheet region-2, B3-beta sheet region-3, B4-beta sheet region-4, B5-beta sheet region-5, and B6-beta sheet region-6). Three regions, collagen triplet, coiled neck, and lectin domain, are distinctly demarcated by the sequences confined within the red arrow marks. The signature sequences within lectin domain are indicated by red horizontal box.