Generation of Dipeptidyl Peptidase-IV-Inhibiting Peptides from β-Lactoglobulin Secreted by Lactococcus lactis
Figure 1
Maps of vectors used in this study. (a) Schematic representation of the secretion vector, pNZ8148#2:SEC. The DNA sequence between the RBS and the stop codon is presented in the rectangle. (b) The codon-optimized BLG gene (537 bp) was cloned into pNZ8148#2:SEC (above) between the KpnI and HindIII restriction enzyme recognition sites, and the resulting plasmid was designated pNZ8148#2:SEC-BLG (below). : nisin-inducible promoter; RBS: ribosome binding site; : sequence of the signal peptide of the usp45 gene product; His-tag: 6x histidine-tag; FXa: factor Xa recognition site; MCS: multiple cloning site; T: terminator; rep: replication gene; cm: chloramphenicol acetyltransferase gene; BLG: β-lactoglobulin.
