Generation of Dipeptidyl Peptidase-IV-Inhibiting Peptides from β-Lactoglobulin Secreted by Lactococcus lactis
Figure 3
Determination of the optimal nisin concentration for secretion of rBLG by NZ9000. (a) Strain NZ9000:SEC-BLG was cultured with various concentrations of nisin (0 to 500 ng/mL). After 3 h of incubation, the OD600 of each sample was measured. The mean and SD from three independent experiments are shown. (b) Protein extracts from cells (CELL, above) or culture supernatants (SUP, below) were analyzed by Western blotting with a 6x His-tag Ab. Representative images from three independent experiments are shown. White and black arrowheads indicate the secretory rBLG precursor (pre-rBLG, 27 kDa) and the secretory form of rBLG (24.3 kDa), respectively.
