Generation of Dipeptidyl Peptidase-IV-Inhibiting Peptides from β-Lactoglobulin Secreted by Lactococcus lactis

Figure 5

Inhibition of DPP-IV activity by trypsin-digested rBLG. Various concentrations of trypsin-digested cBLG (a) or trypsin hydrolysates of 50 μg/mL of either cBLG or purified rBLG (b) were evaluated for inhibitory activity against DPP-IV using a DPP-IV Drug Discovery Kit. Concentrations indicated are the protein concentrations before trypsin treatment. Data are expressed as percent activity remaining in test samples versus that in the control (no sample added). Black, dotted white, and dotted black bars indicate control, cBLG, and purified rBLG (rBLG), respectively. Values represent means and error bars indicate SD (𝑛=3). ^∗𝑃<0.05; ^∗∗𝑃<0.01 versus control.

(a)

(b)