Research Article
ELFN1-AS1: A Novel Primate Gene with Possible MicroRNA Function Expressed Predominantly in Human Tumors
Figure 1
Expression of the ELFN1-AS1 gene in human normal tissues and tumors. (a) Upper pane: PCR with ELFN1-AS1-specific primers. (a) Lower pane: GAPDH control. (a) Lanes: M: DNA size marker; 1: normal brain; 2: normal heart; 3: normal kidney; 4: normal liver; 5: normal lung; 6: normal pancreas; 7: normal placenta; 8: normal skeletal muscle; 9: brain, malignant meningioma, moderately differentiated; 10: lung, nonsmall cell carcinoma; 11: kidney, transitional cell carcinoma, papillary moderately differentiated; 12: kidney, renal cell carcinoma; 13: liver, hepatocellular carcinoma, well differentiated; 14: liver, adenocarcinoma, moderately differentiated; 15: gallbladder, adenocarcinoma; 16: esophagus, squamous cell carcinoma, ulcer, well differentiated; 17: stomach, adenocarcinoma, ulcer, well differentiated; 18: small intestine, mesenchymoma, moderately differentiated; NTC: no template control; PC: positive control. (b) Upper pane: PCR with ELFN1-AS1-specific primers. (b) Lower pane: GAPDH control. (b) Lanes: M: DNA size marker; 1: normal colon; 2: normal ovary; 3: normal peripheral blood leukocytes; 4: normal prostate; 5: normal small intestine; 6: normal spleen; 7: normal testis; 8: normal thymus; 9: colon, adenocarcinoma, moderately differentiated; 10: rectum, adenocarcinoma, moderately/poorly differentiated; 11: ovary, adenocarcinoma, ulcer, moderately differentiated; 12: fallopian tube, medullary carcinoma, poorly differentiated; 13: uterus, adenocarcinoma; 14: ureter, moderately differentiated, transitional cell carcinoma; 15: bladder, transitional cell carcinoma, papillary; 16: testis, germinoma; 17: parotid, squamous cell carcinoma, mixed; NTC: no template control; PC: positive control.
