Effect of Exercise Intensity on Isoform-Specific Expressions of NT-PGC-1α mRNA in Mouse Skeletal Muscle
Figure 2
Isoform-specific expression of NT-PGC-1α-a, NT-PGC-1α-b, and NT-PGC-1α-c and PGC-1α-a, PGC-1α-b, and PGC-1α-c mRNA in response to different intensities of exercise. (a) Induction of NT-PGC-1α-a, NT-PGC-1α-b, and NT-PGC-1α-c mRNA and total NT-PGC-1α mRNA in skeletal muscle (gastrocnemius) at low-, medium-, and high-intensity exercise in C57BL/6J mice. (b) Induction of total NT-PGC-1α protein expression in skeletal muscle (gastrocnemius) in C57BL/6J mice by exercise. C57BL/6J mice were subjected to treadmill running exercise at 20 m/min for 30 min. Gastrocnemius were separated 6 h, 12 h, and 18 h after exercise. Protein expression was analyzed by Western blotting, and β-tubulin was blotted as a loading control. (c) Induction of PGC-1α-a, PGC-1α-b, and PGC-1α-c mRNA and total PGC-1α mRNA in the same tissue and conditions above. Control mice were kept in cage without food. Gastrocnemius were separated 2 h after exercise. Relative gene expression was quantified by real-time PCR and relative to amount of each cDNA, compared to control mice. Values are means ± S.E. (). versus ctrl. Data are representative of three experiments.
