Effect of Exercise Intensity on Isoform-Specific Expressions of NT-PGC-1α mRNA in Mouse Skeletal Muscle
Table 1
Primer sequences for PCR analysis.
Gene
Forward primer
Reverse primer
Total PGC-1
5′-TGC CAT TGT TAA GAC CGA G-3′
5′-GGT CAT TTG GTG ACT CTG G-3′
Total NT-PGC-1
5′-TGC CAT TGT TAA GAC CGA G-3′
5′-GGT CAC TGG AAG ATA TGG C-3′
NT-PGC-1-a
5′-TTG ACT GGC GTC ATT CG-3′
5′-CTG GAA GAT ATG GCA CAT-3′
NT-PGC-1-b
5′-CAT GGA TTC AAT TTT GAA ATG-3′
5′-GGT CAC TGG AAG ATA TGG C-3′
NT-PGC-1-c
5′-CTA TGC TGC TGT GTG CTG-3′
5′-GGT CAC TGG AAG ATA TGG C-3′
PGC-1-a
5′-TTG ACT GGC GTC ATT CG-3′
5′-GGT CAT TTG GTG ACT CTG G-3′
PGC-1-b
5′-CAT GGA TTC AAT TTT GAA ATG-3′
5′-GGT CAT TTG GTG ACT CTG G-3′
PGC-1-c
5′-CTA TGC TGC TGT GTG CTG-3′
5′-GGT CAT TTG GTG ACT CTG G-3′
Glut4
5′-GTT GGT CTC GGT GCT CTT AGT-3′
5′-ATA GCA TCC GCA ACA TAC TGG-3′
ATP5B
5′-GGC ACT GAA GGC TTG GTT AG-3′
5′-CAA GAG AAG ATT CTC AGC GAC-3′
Cyc1
5′-CCC TGA CCT CAG CTA CAT C-3′
5′-CAA GAG AAG ATT CTC AGC GAC-3′
COX5B
5′-GCT GCA TCT GTG AAG AGG ACA AC-3′
5′-CAG CTT GTA ATG GGT TCC ACA GT-3′
MHC I
5′-CCA AGG GCC TGA ATG AGG AG-3′
5′-GCA AAG GCT CCA GGT CTG AG-3′
MHC II a
5′-AAG CGA AGA GTA AGG CTG TC-3′
5′-GTG ATT GCT TGC AAA GGA AC-3′
MHC II b
5′-CGA AGG CGG AGC TAC GGT CA-3′
5′-CGG CAG CCA CTT GTA GGG GT-3′
MHC II x
5′-GCC AGG GTC CGT GAA CTT GAA G-3′
5′-CCT CCG CTT CCT CAG CTT GTC T-3′
IGF-1
5′-AAA GCA GCC CGC TCT ATC C-3′
5′-CTT CTG AGT CTT GGG CAT GTC A-3′
Myostatin
5′-GGC CAT GAT CTT GCT GTA AC-3′
5′-TTG GGT GCG ATA ATC CAG TC-3′
Cyclophilin
5′-CCA TCG TGT CAT CAA GGA CTT CAT-3′
5′-CTT GCC ATC CAG CCA GGA GGT CTT-3′
PGC-1: peroxisome proliferators-activated receptor- coactivator-1; NT-PGC-1: N-truncated PGC-1; Glut4: glucose transporter 4; ATP5B: ATP synthase B subunit; Cyc1: cytochrome C unit 1; COX5B: cytochrome oxidase 5 B subunit; MHC I: myosin heavy chain I; MHC II a: myosin heavy chain II a; MHC II b: myosin heavy chain II b; MHC II x: myosin heavy chain II x; Cyclophilin B: peptidylprolyl isomerase B; IFG-1: insulin-like growth factor 1.
