Attenuation of Collagen-Induced Arthritis in Mice by Salmon Proteoglycan

Figure 2

Administration of PG-attenuated infiltration of macrophages and neutrophils, accumulation of osteoclasts, and chemokine expression in joints of CII-immunized mice. ((a)–(c)) Immunostaining of macrophages, ((d)–(f)) immunostaining of neutrophils, ((g)–(i)) TRAP staining for osteoclasts, ((j)–(l)) quantitative analysis of macrophages, neutrophils, and osteoclasts, and ((m)–(o)) relative mRNA expression of CCL2, CXCL1, and CXCL2 in the joints of CII-immunized mice. Control, CII-PBS, and CII-PG indicate nonimmunized mice, CII-immunized mice administered with PBS, and CII-immunized mice administered with PG, respectively. Single asterisk (𝑃<0.05) and double asterisks (𝑃<0.01) indicate that the value is significantly different.

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