Attenuation of Collagen-Induced Arthritis in Mice by Salmon Proteoglycan
Figure 4
Administration of PG suppressed CII-specific cytokine response of splenocytes but did not alter CII-specific antibody production in serum. ((a)-(b)) CII-specific cytokine response of splenocytes. On day 10 of the first CII-immunization, splenocytes were collected and stimulated with CII ex vivo for 48 h. Titers of IL-17A (a) and IFN-γ (b) were determined from culture supernatants by ELISAs. The results are expressed as the means ± SD of 3 independent experiments. ((c)-(d)) CII-specific antibody production in serum. Sera of CIA mice were collected at the end of experiments. Anti-CII IgG1 (c) and IgG2a (d) levels in serum were determined by ELISA. Control, CII-PBS, and CII-PG indicate nonimmunized mice, CII-immunized mice administered with PBS, and CII-immunized mice administered with PG, respectively. Double asterisk () indicates that the value is significantly different. NS: not significantly different.
