Figure 2: Luciferase reporter assay to determine the regulatory effect of C/EBPα on FTO promoter activity. Three mutant variants (mutated at C/EBP site, Sp1 site, and both of them, resp.) were constructed. Relative luciferase activities (RLU) were measured three independent times in Hela and HEK 293 cells. Results are presented as mean RLU ± SE of three independent experiments (* ).