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Figure 4: Identification of the sequence motif responsible for activation of the FTO promoter by C/EBPα. ChIP assay in HEK 293 cells showing that C/EBPα can bind to the human FTO promoter site. Amounts of coprecipitated DNA (Anti-C/EBPα) and the corresponding amounts in the input chromatin samples (input 1 : 10) were measured by PCR.