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BioMed Research International
Volume 2014, Article ID 408068, 7 pages
Research Article

Inhibitory Effect of Plant Manilkara subsericea against Biological Activities of Lachesis muta Snake Venom

1Laboratório de Venenos e Toxinas de Animais e Avaliação de Inibidores, Departamento de Biologia Celular e Molecular, Instituto de Biologia, Universidade Federal Fluminense, Outeiro de São João Batista, 3º andar, Sala 310, 24020-141 Niterói, RJ, Brazil
2Programa de Pós-Graduação em Ciências Aplicadas a Produtos para a Saúde, Faculdade de Farmácia, Universidade Federal Fluminense, 24220-900 Niterói, RJ, Brazil
3Programa de Pós-Graduação em Biotecnologia Vegetal, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, 21941-599 Rio de Janeiro, RJ, Brazil
4Centro de Pesquisa e Desenvolvimento, Fundação Ezequiel Dias, 30510-010 Belo Horizonte, MG, Brazil

Received 28 August 2013; Revised 9 December 2013; Accepted 10 December 2013; Published 8 January 2014

Academic Editor: Leonardo A. Calderon

Copyright © 2014 Eduardo Coriolano De Oliveira et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Snake venom is composed of a mixture of substances that caused in victims a variety of pathophysiological effects. Besides antivenom, literature has described plants able to inhibit injuries and lethal activities induced by snake venoms. This work describes the inhibitory potential of ethanol, hexane, ethyl acetate, or dichloromethane extracts and fractions from stem and leaves of Manilkara subsericea against in vivo (hemorrhagic and edema) and in vitro (clotting, hemolysis, and proteolysis) activities caused by Lachesis muta venom. All the tested activities were totally or at least partially reduced by M. subsericea. However, when L. muta venom was injected into mice 15 min first or after the materials, hemorrhage and edema were not inhibited. Thus, M. subsericea could be used as antivenom in snakebites of L. muta. And, this work also highlights Brazilian flora as a rich source of molecules with antivenom properties.