Inhibitory Effect of Plant Manilkara subsericea against Biological Activities of Lachesis muta Snake Venom
Figure 2
Effect of M. subsericea extracts on clotting induced by L. muta venom. L. muta venom (25 μg/mL) was incubated for 30 minutes with M. subsericea extracts (250 μg/mL); then mixtures were added to plasma and clotting monitored, as described in the Methods. Columns are L. muta venom incubated with NaCl (C1); with DMSO (C2), with ethanolic extract of stem (1) or leaves (2); with hexane- (3) or ethyl acetate- (4) soluble fractions of stem; with hexane- (5) or ethyl acetate- (6) soluble fraction of leaves. Data are expressed as means ± S.E.M of three individual experiments (). Significance level () when compared to C1 or C2 columns. # means that plasma did not clot until 600 seconds of observation.
