Research Article
Purification and Characterization of Catalase from Marine Bacterium Acinetobacter sp. YS0810
Table 1
Purification of YS0810CAT (100 mL fermentation broth).
| Purification steps | Total activity (U) | Total protein (mg) | Specific activity (U/mg) | Yield (%) | Purification (fold) |
| Cell extracts | 681889.741 | 914.38 | 745.74 | 100 | 1 | Ethanol precipitation | 616486.03 | 407.23 | 1513.85 | 90.41 | 2.03 | DEAE Sepharose | 374847.06 | 26.68 | 14049.74 | 54.97 | 18.84 | Superdex 200 | 115496.483 | 4.13 | 27965.25 | 16.94 | 37.50 | Resource Q | 47021.00 | 1.26 | 37318.25 | 6.9 | 50.04 |
|
|