Periodontal Ligament Mesenchymal Stromal Cells Increase Proliferation and Glycosaminoglycans Formation of Temporomandibular Joint Derived Fibrochondrocytes

Figure 4

Conditioned medium increases chondrogenic gene expression. Real time qPCR for collagen type I (a), collagen type II (b), Sox 9 (c), and Aggrecan (d) was performed on RNA samples extracted from pellets cultured either in expansion medium or conditioned medium for 3 weeks . Geometric average of GAPDH and β-actin was used for normalization. Expression level in pellets cultured in expansion medium was chosen as reference. values were calculated with Student’s t-test.

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