Ex Vivo Expansion of Functional Human UCB-HSCs/HPCs by Coculture with AFT024-hkirre Cells

Figure 1

Reconstruction of AFT024-hKirre cells and expression of hKirre-GFP protein. (a) Recombinant hkirre-GFP retrovirus vector design: LTR indicates long terminal repeat; puro, puromycin-resistant gene; PCMV IE, cytomegalovirus (CMV) immediate early promoter; hKirre-GFP, human Kirre attached with GFP protein. (b) AFT024 cells transfected with mock plasmid (×400). (c) AFT024 cells transfected with hkirre plasmid (×400). (d) AFT024-hkirre cells (×100). Images (b) to (d) showed a high transfection efficiency under fluorescence microscope. (e) Western Blot analysis of hkirre expression in the AFT024 transformed cells and control cells. (f) Total AFT024-hkirre cells lysate was checked for the hkirre protein on SDS page and stained with commassive brilliant blue (stain). A differentially expressed protein of approximately 89 kDa was detected (A) compared with the control cells (B).