Ex Vivo Expansion of Functional Human UCB-HSCs/HPCs by Coculture with AFT024-hkirre Cells

Table 1

Umbilical cord blood HSCs expansion over seven days of culture.

Groups	AFT024-hKirre cells	AFT024 control cells	Stroma free (cytokines added)	Stroma free (without cytokines)
CD34^+ cells	14.87 ± 2.54*	7.88 ± 0.74*	15.54 ± 5.35	2.86 ± 1.19
CD34^+CD38^− cells	60.47 ± 20.52*	24.32 ± 8.83*	14.91 ± 2.73	0.93 ± 0.23
CD34^+CD38^+ cells	45.15 ± 21.28	41.73 ± 10.58	58.60 ± 13.46	8.84 ± 5.34

Cells were analyzed by flow cytometry after staining with PE-conjugated mAb CD34 and FITC-conjugated mAb CD38. Values indicate fold increase based on the initial number of cells (1 × 10⁵). The results are expressed as mean ± SD (𝑛=5). *𝑃<0.05 versus control cells (two-tailed Student’s 𝑡-test).