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BioMed Research International
Volume 2014, Article ID 412570, 10 pages
Research Article

Different Effects of Implanting Sensory Nerve or Blood Vessel on the Vascularization, Neurotization, and Osteogenesis of Tissue-Engineered Bone In Vivo

1Department of Orthopaedics, Xijing Hospital, Fourth Military Medical University, Xi’an 710032, China
2Department of Orthopaedics, Longgang District Central Hospital of Shenzhen, Shenzhen 518116, China
3Department of Orthopaedics, People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning 530021, China

Received 29 March 2014; Accepted 2 June 2014; Published 30 June 2014

Academic Editor: Zhi-Yong Zhang

Copyright © 2014 Jun-jun Fan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


To compare the different effects of implanting sensory nerve tracts or blood vessel on the osteogenesis, vascularization, and neurotization of the tissue-engineered bone in vivo, we constructed the tissue engineered bone and implanted the sensory nerve tracts (group SN), blood vessel (group VB), or nothing (group Blank) to the side channel of the bone graft to repair the femur defect in the rabbit. Better osteogenesis was observed in groups SN and VB than in group Blank, and no significant difference was found between groups SN and VB at 4, 8, and 12 weeks postoperatively. The neuropeptides expression and the number of new blood vessels in the bone tissues were increased at 8 weeks and then decreased at 12 weeks in all groups and were highest in group VB and lowest in group Blank at all three time points. We conclude that implanting either blood vessel or sensory nerve tract into the tissue-engineered bone can significantly enhance both the vascularization and neurotization simultaneously to get a better osteogenesis effect than TEB alone, and the method of implanting blood vessel has a little better effect of vascularization and neurotization but almost the same osteogenesis effect as implanting sensory nerve.