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BioMed Research International
Volume 2014 (2014), Article ID 413575, 5 pages
http://dx.doi.org/10.1155/2014/413575
Research Article

Anticancer Activity of Marine Sponge Hyrtios sp. Extract in Human Colorectal Carcinoma RKO Cells with Different p53 Status

1College of Pharmacy, Duksung Women’s University, No. 33, 144-gil, Samyang-ro, Dobong-gu, Seoul 132-714, Republic of Korea
2Korea Institute of Ocean Science & Technology, 787 Haeanro, Ansan 426-744, Republic of Korea

Received 20 June 2014; Revised 9 August 2014; Accepted 11 August 2014; Published 27 August 2014

Academic Editor: Shun-Fa Yang

Copyright © 2014 Hyun Kyung Lim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Drug development using marine bioresources is limited even though the ocean occupies about 70% of the earth and contains a large number of biological materials. From the screening test of the marine sponge extracts, we found Hyrtios sp. sponge collected from Chuuk island, Micronesia. In this study, the Hyrtios sp. extract was examined for anticancer activity against human colorectal carcinoma RKO cells that are wildtype for p53 and RKO-E6 that are p53 defective. The Hyrtios sp. extract dose-dependently inhibited viability in both cell lines. Multinucleation as an indication of mitotic catastrophe was also observed. Cytotoxicity tests gave significantly different results for RKO and RKO-E6 cells after 48 h exposure to Hyrtios sp. extract. In RKO cells treated with Hyrtios sp. extract, cell death occurred by induction of p53 and p21 proteins. In p53-defective RKO-E6 cells, Hyrtios sp. extract decreased expression of JNK protein and increased p21 protein. These results indicate that Hyrtios sp. extract induced apoptosis via different pathways depending on p53 status and could be a good natural product for developing new anticancer drugs.