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413708.fig.002b
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Figure 2: Characterization of Bovine Endothelial and Smooth Muscle Cells. The isolated cells were verified with the EC specific markers VEGFR2, VE-cadherin, PECAM-1 and the SMC specific markers calponin, SMA-α, MYH-11 by RT-PCR (a) and Western blot (b). GAPDH served as internal control. The endothelial (c) and smooth muscle cells (d) were also identified with the above-mentioned markers via fluorescent staining. The isolated endothelial cells were further examined for the typical endothelial activity of LDL up-take (e). All pictures are representative of one cow sample out of three.