Table of Contents Author Guidelines Submit a Manuscript
BioMed Research International
Volume 2014, Article ID 415375, 11 pages
Research Article

CCR7 Regulates Cell Migration and Invasion through JAK2/STAT3 in Metastatic Squamous Cell Carcinoma of the Head and Neck

1Department of Oromaxillofacial-Head & Neck Surgery and Department of Oral and Maxillofacial Surgery, School of Stomatology, China Medical University, No. 117 Nanjing Bei Jie, Heping District, Shenyang, Liaoning 110002, China
2Department of Head and Neck, Henan Tumor Hospital, Zhengzhou University, 127 Dongming Road, Zhengzhou, Henan 450008, China

Received 8 July 2014; Accepted 27 August 2014; Published 27 October 2014

Academic Editor: Sung-Hoon Kim

Copyright © 2014 Fa-Yu Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Squamous cell carcinoma of the head and neck (SCCHN) frequently involves metastasis at diagnosis. Our previous research has demonstrated that CCR7 plays a key role in regulating SCCHN metastasis, and this process involves several molecules, such as PI3K/cdc42, pyk2, and Src. In this study, the goals are to identify whether JAK2/STAT3 also participates in CCR7’s signal network, its relationship with other signal pathways, and its role in SCCHN cell invasion and migration. The results showed that stimulation of CCL19 could induce JAK2/STAT3 phosphorylation, which can be blocked by Src and pyk2 inhibitors. After activation, STAT3 was able to promote low expression of E-cadherin and had no effect on vimentin. This JAk2/STAT3 pathway not only mediated CCR7-induced cell migration but also mediated invasion speed. The immunohistochemistry results also showed that the phosphorylation of STAT3 was correlated with CCR7 expression in SCCHN, and CCR7 and STAT3 phosphorylation were all associated with lymph node metastasis. In conclusion, JAk2/STAT3 plays a key role in CCR7 regulating SCCHN metastasis.