A Novel Laccase with Potent Antiproliferative and HIV-1 Reverse Transcriptase Inhibitory Activities from Mycelia of Mushroom Coprinus comatus

Table 4

Effect of metal ions and EDTA on C. comatus laccase activity.

Metal ions	Residual activity (% of control)
	1.25 mM	2.5 mM	5 mM	10 mM
Al^3+	89.1	88.5	85.4	84.3
Ca^2+	86.0	83.8	80.6	69.7
Co^2+	100	100	100.9	89.9
Cu^2+	98.0	100	110.8	110.4
Fe^2+	34.2	28.1	22.1	10.7
Hg^2+	82.3	71.2	61.8	45.5
K^+	98.0	106.4	97.8	91.0
Li^+	85.3	86.8	87.4	83.0
Mg^2+	99.7	100	86.8	80.9
Mn^2+	94.1	91.1	85.8	70.0
Zn^2+	100	98.9	90.0	88.0
EDTA	98.9	100	98.0	97.5

Laccase activity in the absence of metal ions was regarded as 100%.