Proteinases in Excretory-Secretory Products of Toxocara canis Second-Stage Larvae: Zymography and Modeling Insights

Figure 1

Electrophoretic detection of bands with proteolytic activity in TES of T. canis at different pH values.Samples of TES were loaded at a quantity of 10 μg/well in slab gels of 10% (w/v) acrylamide copolymerized with 0.1% (w/v) gelatin. After electrophoretic separation, proteinase activity was developed using acetate buffer (pH 5.5, lane 2), phosphate buffer (pH 7.6, lane 3), or glycine buffer (pH 9.0, lane 4) and then gels were stained with Coomassie blue. In other assays, electrophoresis of TES was performed in 10% (w/v) acrylamide gels and protein bands were directly stained with Coomassie blue (lane 1). Molecular weight of bands with gelatinolytic activity is indicated at the right of the corresponding lanes.