Proteinases in Excretory-Secretory Products of Toxocara canis Second-Stage Larvae: Zymography and Modeling Insights

Figure 3

Proteolytic activity towards different substrates by TES components from T. canis at different pH values. TES samples (10 μg/well) were electrophoretically separated in slab gels of 10% (w/v) acrylamide copolymerized with the following substrates: 0.5 mg/gel laminin, 0.5 mg/gel fibronectin, 0.1% (w/v) bovine serum albumin, and 0.1% (w/v) goat IgG. After electrophoresis, proteinase activity was developed using acetate buffer (pH 5.5), phosphate buffer (pH 7.6), or glycine buffer (pH 9.0) and then gels were stained with Coomassie blue. Molecular weight of bands with activity is indicated at the right of the panels indicated at the top. The pH values used are indicated at the bottom of each lane.