BioMed Research International / 2014 / Article / Fig 4

Research Article

Proteinases in Excretory-Secretory Products of Toxocara canis Second-Stage Larvae: Zymography and Modeling Insights

Figure 4

Densitometry analyses of representative proteolytic activities towards different substrates by TES components from T. canis L2s. TES samples previously separated by substrate gel electrophoresis were selected and densitometry analyses were carried out on tag image format file (TIFF) images delimiting each band with noticeable proteolytic activity from surrounding background zones and separate density determinations were obtained. Samples shown correspond to TES components resolved in 10% (w/v) acrylamide copolymerized with (a) 0.1% (w/v) gelatin at pH 7.6, (b) 0.5 mg/gel laminin at pH 9.0, (c) 0.5 mg/gel fibronectin at pH 9.0, and (d) 0.1% (w/v) bovine serum albumin at pH 9.0. The MW (in kDa) of each peak of density is indicated.

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