α-Actinin TvACTN3 of Trichomonas vaginalis Is an RNA-Binding Protein That Could Participate in Its Posttranscriptional Iron Regulatory Mechanism

Figure 3

Putative functional domains present in TvACTNs of T. vaginalis. Principal motifs of the deduced amino acid sequences of the five tvactn genes reported in the T. vaginalis genome sequence [29]. TvACTN1 (609 aa), TvACTN2 (931 aa), TvACTN3 (1129 aa), TvACTN4 (1137 aa), and TvACTN5 (1271 aa). By SMART, MOTIF SCAN, and PROSITE programs, we found the principal motif sequences present in the five TvACTNs proteins, two calponin domains (CH) at the N-terminus followed by variable spectrin repeats (SR) in the central rod domain, and two EF-hands: EF-hand (E) and calcium-insensible EF-hand (EF) at the C-terminus. TvACTN4 and TvACTN5 present more divergent central region and C-terminus. TvACTN4 has one coiled coil (C) and nine SEL domains (S), and TvACTN5 presents seven C2 domains (C2).