α-Actinin TvACTN3 of Trichomonas vaginalis Is an RNA-Binding Protein That Could Participate in Its Posttranscriptional Iron Regulatory Mechanism
Figure 7
Production of polyclonal antibodies and specific recognition of TvACTN3 domains (DIr, DIIr, and DIIIr) and T. vaginalis cytoplasmic proteins by the specific antibodies. WB assays using (a) TvACTN3r; (b) DIr, (c) DIIr, (d) DIIIr purified recombinant proteins; and (e) cytoplasmic proteins from T. vaginalis grown in regular iron conditions used as antigens and transferred onto NC membranes and incubated with α-TvACTN3r, α-DIr, α-DIIr, and α-DIIIr polyclonal antibodies (lanes 1–4). kDa, molecular weight markers in kilodaltons (Bio-Rad).
