New Researches and Application Progress of Commonly Used Optical Molecular Imaging Technology
Figure 5
Bioluminescence imaging of regional and cellular localization of CuZnSOD in the PVN. (a) Map of the location of microinjection sites for AdLacZ (left, O) and AdCuZnSOD (left, E) in the PVN relative to the third ventricle by 4.5 mm (ventral), 0.7 mm (posterior), and 0.3 mm (lateral). Subpopulations of PVN nuclei are outlined: mm, medial magnocellular; mpd, medial parvocellular; pml, posterior magnocellular; pv, parvocellular; 3V, third ventricle. Representative photomicrographs of coronal brain sections showing CuZnSOD immunoreactivity in the PVN of AdCuZnSOD- or control-treated mice are shown on the right. (b) Representative confocal images of coronal brain sections showing CuZnSOD immunoreactivity (left), glial fibrillary acidic protein (GFAP), or neuronal nuclei (NeuN; center) and merged images (right). Adapted from Burmeister et al. [50].
