BioMed Research International / 2014 / Article / Fig 3

Research Article

Anticancer Drug 2-Methoxyestradiol Protects against Renal Ischemia/Reperfusion Injury by Reducing Inflammatory Cytokines Expression

Figure 3

2ME2 reduced HIF-1α, BNIP3, caspase-3, Bcl-2, TNF-α, and IL-1β expression. Gel photographs depicting mRNA expression of HIF-1α, BNIP3, caspase-3, Bcl-2, and the cytokines TNF-α and IL-1β, using RT-PCR in renal tissue extracts from mice treated with saline or I/R-only or I/R+2ME2 (10 mg/kg) or I/R+2ME2 (20 mg/kg). β-Actin was the internal control. The ratios of HIF-1α, BNIP3, caspase-3, Bcl-2, TNF-α, and IL-1β to β-actin are shown (numbers above the top three panels), respectively. Data are representative of three independent experiments.
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